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Continual passage of Staphylococcus epiderm/dis in subinhibitory levels of the biocide triclosan results in a marked increase in the minimum inhibitory concentration, antibiotic resistance, and ethidium bromide resistance

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"Triclosan is a multi-purpose biocide that is used in many personal care products, including antibacterial handsoaps and toothpastes. The wide usage oftriclosan fosters its dispersal into the enviro11l11ent, which might contribute to the ability of microorganisms to become resistant to triclosan in addition to certain other biocides and clinical antibiotics. The aim of this study was to evaluate whether long-term exposure of two strains of Staphylococclls epide]'midis to subinhibitory concentrations oftriclosan would select for resistant mutants, and whether their ability to form polysaccharide biofilms lends to this resistance. This study also aimed to detelmine whether a mutation in the triclosan target was responsible for resistance, and to determine whether these mutants could exhibit cross-resistance to chlorhexidine and clinical antibiotics. In addition, efflux capability was assessed as a presumable resistance mechanism."

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